Somatic embryogenesis in (Anthurium andraeanum Lind.) variety ‘LAMBADA’
DOI:
https://doi.org/10.35197/rx.04.01.2008.08.nrKeywords:
culture medium, growth regulators, somatic embryosAbstract
Various concentrations and types of growth regulators were tested to induce indirect somatic embryogenesis in Anthurium andraeanum Lind. (Monocot). For callus formation, leaf segments from micropropagated plants were used as explants on a modified MS culture medium supplemented with 6.79 µM 2,4-dichlorophenoxyacetic acid (2,4-D) and 2.32 µM kin (kinetin). Differentiation of somatic embryos was achieved in the modified MS culture medium containing 4.44 µM 6-benzylaminopurine (6-BAP), under dark conditions. Germination of somatic embryos was obtained in the same culture medium tested, supplemented with 1.78 µM 6-BAP when placed in a growth chamber with sunlight.
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Copyright (c) 2008 Nydia del Rivero Bautista, Daniel Agramonte Peñalver, Raúl Barbón Rodríguez, Wilder Camacho Chiu, Raúl Collado López, Felipe Jiménez Terry, Marta Pérez Peralta , Odalys Gutiérrez Martínez
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